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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Eur J Immunol. 2016 Jun 8;46(8):1948–1958. doi: 10.1002/eji.201646395

Figure 5. ADRB2 signaling does not affect CD8+ T-cell proliferation or programming.

Figure 5

(A) Splenocytes from ADRB2-sufficient or -deficient Cl4 mice were isolated and incubated for 3 days in the presence or absence of the HA peptide IYSTVASSL and NE. Proliferation was measured by dilution of a proliferation dye by flow cytometry. (B) Human CD8+CD45RA+ cells were purified from peripheral blood and activated with plate-bound anti-CD3 and anti-CD28 antibodies, either neutralizing cytokines (Neut) or in the presence of rhIL-12 (IL-12) and in the presence or absence of NE for 7 days. On day 8, cells were restimulated with plate-bound anti-CD3 for 24 hours. IFN-γ and TNF-α in the supernatant were measured by ELISA. Data are presented as mean +/− SEM of triplicate determinations and are representative of (A) 3 experiments with at least 1 mouse in each experiment, and (B) 2 independent experiments from separate donors. Comparisons of NE treatments to controls were not significant as assessed by 2-way ANOVA with Bonferroni post-test.