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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Eur J Immunol. 2016 Jun 8;46(8):1948–1958. doi: 10.1002/eji.201646395

Figure 6. ADRB2 signaling modulates cytokine secretion programming in mouse CD8+ T cells in vivo.

Figure 6

(A) OT-I T cells were transferred (2000/recipient) into naïve C57Bl/6 mice followed by infection with 106 PFU VSV-OVA the next day (day 0). DMSO (V.C.) or Salmeterol was given i.p. on days 0, 1 and 2. (B) Spleens were harvested on d7, and OT-I-specific cellular expansion was determined by flow cytometry. Data display percent of Kb-tet+ cells of the total CD8+ T-cell pool (left) and the total numbers of Kb-tet+ cells within the spleen (right). (C) IFN-γ and TNF-α secretion were measured from splenocytes by flow cytometry after 24 hours restimulation with SIINFEKL peptide. Data are presented as mean +/− SEM of triplicate determinations and are representative of 2 independent experiments with 4 mice per treatment group in each experiment.. *p<0.05 compared to V.C, 1-way ANOVA with Bonferroni post-test.