Table 2.

Summary of PCR product sequencing results

PCR product	Sequence result
PCR 1	Identical with WT Ad5
PCR 2	Generated only
PCR 2′	Generated only
PCR 3	Identical with WT Ad5
PCR 4	Identical with AdhAQP1
PCR 5	Identical with AdhAQP1
PCR 6	Identical with WT Ad5
PCR 7	Unable to generate

All generated products, except PCR 2 and PCR 2′, were isolated from agarose gels and subjected to conventional sequencing. PCR 2 and PCR 2′ were, in effect, the templates for the PCR reactions used to generate PCR 3 and PCR 4.