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. Author manuscript; available in PMC: 2017 Jan 17.
Published in final edited form as: Biol Psychiatry. 2014 Jul 15;77(4):335–344. doi: 10.1016/j.biopsych.2014.07.008

Figure 5.

Figure 5

Effects of neonatal maternal separation (NMS) on methylation and methylation-dependent messenger RNA (mRNA) expression of retinoic acid receptor (RAR)α in adult dentate gyrus-derived neural precursor cells in vitro. (A) NMS increased the ratio of methylated (M) fraction to unmethylated fraction (U) of RARα promoter (t4 = 2.956, p < .05). (B) The DNA methyltransferase inhibitor 5-aza-dC (10 μmol/L) increased expression of RARα mRNA in adult dentate gyrus-derived neural precursor cells from the animal facility reared (AFR) and NMS groups (AFR vs. NMS, F1,20 = 67.160, p < .0001; vehicle [V] vs. 5-aza-dC, F1,20 = 39.090, p < .0001; interaction, F1,20 = .780, n.s.). Post hoc comparison of vehicle-treated AFR and 5-aza-dC-treated NMS groups showed that the groups did differ. Data are shown as the means ± SEM. Statistical significance is shown at *p < .05, **p < .01, ***p < .001, or ****p < .0001. n.s., no statistically significant difference.