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. Author manuscript; available in PMC: 2017 Jan 17.
Published in final edited form as: Mol Pharm. 2016 Apr 28;13(6):2010–2025. doi: 10.1021/acs.molpharmaceut.6b00140

Figure 5.

Figure 5

DNA damage in GSTA1 overexpressing cells after ANS–DOX and DOX exposure.(A) Activation of DNA damage signaling was assessed by phosphorylation of p53 at Ser15 and Histon-γH2AX at Ser139 using Western blot. Cdk2 was used as a loading control. GSTA1 overexpressing and vector control cells were treated with 0.2 μM DOX or 2 μM ANS–DOX for the indicated time periods or for 3 h with the indicated concentration. (B) DOX and ANS–DOX mediated DNA damage in GSTA1 overexpressing (light gray) and vector control cells (dark gray) were determined after a 3 h exposure by comet assay. Experiments were performed in triplicate, and data points represent means ± SEM **p < 0.01, ***p < 0.001 as compared with control levels by using a two-tailed student t test.