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. Author manuscript; available in PMC: 2017 Mar 2.
Published in final edited form as: Chembiochem. 2016 Feb 4;17(5):394–397. doi: 10.1002/cbic.201500624

Table 2.

Substrate scope for most selective variants from the P450 library.

graphic file with name nihms841851u2.jpg
Yield TTN[a] dr [cis:trans] ee cis [%][b] ee trans [%][b]
4a
hemin 16 79 22:78 0 −3
P450BM3-T268A 68 342 1:99 33 93
P450PikC-T247A 50 248 25:75 11 −49
P450BM3-CIS-T438S 19 95 8:92 8 90
P450eryF (A245) 30 149 42:58 −5 −37
P450BioI-T238A 54 271 87:13 −96 8
CYP142-T234A 47 234 89:11 94 0
4b
hemin 18 90 17:83 0 n.d.[c]
P450BM3-T268A 65 326 1:99 −40 n.d.[c]
P450PikC-T247A 55 276 11:89 −4 n.d.[c]
P450BM3-CIS-T438S 61 305 75:25 −79 n.d.[c]
P450eryF (A245) 41 204 73:27 −93 n.d.[c]
P450BioI-T238A 43 214 66:34 88 n.d.[c]
CYP142-T234A 48 241 91:9 −96 n.d.[c]
4c
hemin 6 30 13:87 −1 n.d.[c]
P450BM3-T268A 39 195 13:87 −36 n.d.[c]
P450PikC-T247A 41 205 9:91 0 n.d.[c]
P450BM3-CIS-T438S 59 297 35:65 71 n.d.[c]
P450eryF (A245) 17 85 73:27 −81 n.d.[c]
P450BioI-T238A 14 70 30:70 59 n.d.[c]
CYP142-T234A 34 171 90:10 −96 n.d.[c]
[a]

TTN =total turnover number.

[b]

Stereochemistry unassigned; negative sign indicates opposite enantiomer was formed.

[c]

Not determined; baseline separation could not be achieved.

TTNs and stereoselectivities were determined by chiral GC analysis.