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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Endocrinol. 2016 Dec 15;232(3):377–389. doi: 10.1530/JOE-16-0471

Figure 5. Neonatal growth hormone (GH) imprints adult growth hormone regulation of STAT5b activation, nuclear translocation and binding to the CYP2C11 promoter.

Figure 5

(Left Panel) Nuclear levels of activated STAT5b (pSTAT5b) in cultured primary hepatocytes exposed to either the episodic male-like GH profile or the episodic profile containing GH vehicle alone for 6 days. Results are presented as a percentage of nuclear pSTAT5b in hepatocytes exposed to vehicle arbitrarily designated as 100%. Values are means ± SD of at least 6 rats/data point. *P<0.01 vs hepatocytes exposed to the episodic vehicle GH profile from rats of the same neonatal treatment, i.e. CONTROL, MSG, GH or MSG+GH as described in Figure 1.

(Right Panel) Representative ChIP blots (2 of 5/group) demonstrating episodic GH regulation (♂GH) described above, of pSTAT5b binding to the Cyp2c11 promoter in hepatocytes derived from adult male rats neonatally treated with either diluents (CONTROL), MSG, GH or MSG+GH as described in Figure 1.