Figure 4. ¹¹C-acetate uptake in bone-localized MM.

A) (i.) ¹¹C-acetate PET/CT image of a KaLwRij mouse bearing i.v. 5TGM1 MM cells (21 days post tumor inoculation); (ii.) ¹¹C-acetate PET/CT image of a control KaLwRij mouse (iii) TAC of MM (red) and control (blue) bones. ROIs were defined around the tibial diaphysis. ** p<0.01 by ANOVA.

B) SUVs from the tibial ROI of 5TGM1 iv injected KaLwRij mice vs. controls (n=4). C) ¹¹C-acetate PET/CT image of1 year old KaLwRij mice, without (i., control) or with sustained M-component at the SPEP (ii. Right, MM). D) TAC for the tibial diaphysis ROI shows significantly higher uptake in the mouse with gammopathy (MM). E) Flow cytometry analysis of bone marrow and spleen homogenate upon staining with anti-CD138 reveals pathologic elevation of plasma cell counts (CD138⁺ cells) in the spleen and bone marrow in the aged mouse with gammopathy (red), relative to the SPEP-negative KaLwRij (blue) or C57/BL6 (green) age-matched controls, consistent with MM. *p<0.05, ** p<0.01