Figure 5.

Rhythmic Nuclear Phosphorylation and Inferred Kinase Activities

(A) Heatmap representation of rhythmically phosphorylated peptides (n = 92, right panel) with associated non-rhythmic nuclear proteins (left panel).

(B and C) Phase (B) and peak-to-trough (C) amplitude (log₂) distributions for rhythmic nuclear phosphoproteins in (A).

(D) Individual examples of rhythmic phosphorylated sites (blue line, nuclear proteins; green line, nuclear phosphosites).

(E) Kinase motifs displaying rhythmic activities inferred from non-rhythmic nuclear proteins containing rhythmic phosphorylation sites through a linear model with elastic-net regularization. Direction of lines indicates peak activity times, and distances to the center of solid lines indicate activity amplitudes of each motif.

(F) Inferred rhythmic activities of CSNK1A1, GSK3A, and MAPK14 compared with their respective nuclear protein accumulations.