Figure 4.

Exercise Induces TFEB Nuclear Translocation

(A) GCN tissue sections of TFEB KO and WT mice were immunostained with anti-TFEB antibody and counter-stained with hematotoxylin as indicated. Arrows indicate nuclear localization of endogenous TFEB in WT mice after an acute bout of exercise. The scale bars represent 50 μm.

(B) Endogenous TFEB was immunostained in muscle cryosections of sedentary, 4 days mild exercised, and 7 weeks intense trained WT mice. Arrows indicate TFEB nuclear localization.

(C) Expression analysis of genes related to mitochondrial biogenesis and mitochondrial respiratory chain in WT skeletal muscle before and after acute exercise. Data are shown as mean ± SE, n = 3; ^∗p < 0.05, ^∗∗p < 0.01.