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. 2017 Jan 10;25(1):182–196. doi: 10.1016/j.cmet.2016.11.003

Figure 7.

Figure 7

TFEB Also Controls Glucose Uptake via Regulation of AMPK Signaling Pathways

(A) Quantitative real-time PCR of nNOS transcript in WT (white bar), TFEB-overexpressing (black), and TFEB KO muscles (gray). Values are expressed as fold induction compared to controls. Data are shown as mean ± SE, n = 8; ∗p < 0.05, ∗∗p < 0.01.

(B) Western blot of nNOS and densitometric quantification. Value are normalized for GAPDH; ∗p < 0.05; ∗∗∗p < 0.001.

(C) ChIP analyses of muscles from TFEB transgenic and WT mice on nNOS, GLUT1, and GLUT4 promoters. The histograms show the amount of immunoprecipitated DNA as detected by quantitative real-time PCR assay. Data represent mean ± SE of three independent experiments; ∗p < 0.05.

(D) Western blot of AMPK, PAMPK, PACC, and PAKT performed on protein extracts from GCN muscles of AAV2.1-TFEB transfected, TFEB KO, or WT mice. Representative images (left) and densitometric quantification (right) are shown; ∗p < 0.05.