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. Author manuscript; available in PMC: 2018 Jan 23.
Published in final edited form as: Vaccine. 2016 Dec 29;35(4):639–646. doi: 10.1016/j.vaccine.2016.12.008

Figure 2.

Figure 2

Airway mucosal T-cell response magnitude. (A) Experimental vaccination scheme. Sixteen rhesus macaques were immunized with three DNA primes encoding Env at one-month intervals. Eight weeks after the last DNA prime, a mixture of two rAd5 viruses encoding Env and GagPol immunogens was administered IN, IM, AE 4µm, or AE 10µm (n=4 per group). Antigen-specific T-cells were measured throughout the vaccination regimen in BAL by in vitro peptide stimulation and intracellular Th1 cytokine staining and flow cytometric quantitation. Unprimed GagPol-specific (B) and DNA-primed Env-specific (C) CD4+ (top) and CD8+ (bottom) T-cell responses are shown for each vaccination group at the indicated time point. Statistically significant (p<0.05, Wilcoxon rank-sum test) responses relative to the pre-immune measurement for each vaccine group are indicated. (D) Direct comparison of DNA-primed and unprimed BAL responses four weeks after rAd5 vaccination (week 20) is shown.