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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: Clin Cancer Res. 2016 Jul 19;23(2):489–502. doi: 10.1158/1078-0432.CCR-16-0004

Figure 4. Human NK cells secrete high levels of IFN-γ and NK culture supernatants stimulate the migration of T cells.

Figure 4

The KRAS wild-type BxPc3 (A) and KRAS mutant AsPc1 and MiaPaCa2 (B) cell lines were cultured with human NK cells in an in vitro tumor co-culture assay. Control conditions consisted of tumor cells and NK cells cultured with medium alone, IgG alone, cetuximab alone, IL-21 alone or the combination of IgG and IL-21. Culture supernatants were harvested at 48 hrs and analyzed for IFN-γ by ELISA. (C) Chemotaxis of activated T cells in response to culture supernatants derived from NK cells costimulated with cetuximab-coated KRAS wild-type BxPc3 or KRAS mutant AsPc1 tumor cells and IL-21. Culture medium supplemented with monokine induced by gamma-interfereon (MIG) was included as a positive control. Each graph depicts the results from one representative donor ± SD. Three normal donors were tested per cell line. The asterisk (*) denotes p<0.001 versus all conditions shown.