Skip to main content
. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: Clin Cancer Res. 2016 Jul 19;23(2):489–502. doi: 10.1158/1078-0432.CCR-16-0004

Figure 6. NK cell signal transduction is enhanced by IL-21 receptor and Fc receptor engagement by cetuximab-coated mutant KRAS AsPc1 tumor cells.

Figure 6

Purified human NK cells were stimulated with 10 ng/ml of IL-21 and co-cultured with cetuximab-coated KRAS mutant AsPc1 pancreatic cancer cells for 30 mins. Control conditions consisted of media alone, IL-21-stimulated NK cells alone, or cetuximab-coated tumor cells alone. Following incubation, cells were collected and underwent dual flow cytometry staining using anti-CD56 and (A) anti-phospho-ERK1/2 or (B) anti-phospho-STAT1 antibodies. Percentages reported are of dual positive populations (Q2). Each plot depicts the results from one representative donor. Three normal donors were tested per cell line.