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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2016 Jun 10;12(1):120–132. doi: 10.1007/s11481-016-9688-3

Figure 2. Nano-bound Beclin1 siRNA does not compromise the physiological function of the artificial blood brain barrier.

Figure 2

Transendothelial electrical resistance (TEER) across the brain microvascular endothelial cell monolayer of the BBB model was measured following the indicated treatments at the indicated time points. Error bars show the SEM for 3 independent experiments (A). Expression of occludin protein was detected in brain microvascular endothelial cells by immunocytochemistry following the indicated treatments for 24 hours. Cells were immunolabeled with an antibody to occludin (green) and DAPI (blue) staining was used to label cell nuclei. Representative images were acquired at 20X magnification (B).