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Physical localization of the region comprising the cult polymorphic site on carrot chromosomes analyzed by fluorescence in situ hybridization. Pachytene chromosomes were stained with DAPI (blue). Green and red signals represent the physical positions of DHBAC.b0022A10 (mapping to a distal position on the long arm of chromosome 2; Iorizzo et al., 2016) and long-range PCR amplicons spanning the cult region, respectively. Bar = 5 μm.
