Table 2.

Average per molecule excess chemical potential ${\mu }_{ex}^{pw}$ and hydration free energy $\Delta {\mu }_{ex}={\mu }_{ex}^{pw}-{\mu }^{bulk}\left({\mu }^{bulk}=-9.0\left(0.1\right)kcal/mol\right).$ The number in parenthesis indicate statistical error, estimated from propagation of standard deviations. The number of long residence water molecules residing simultaneously in the interior of the proteins at T=300 K are indicated by 〈n_w〉, the drop of internal hydration due to temperature increase is referred as Δn_w = 〈n_w〉³⁰⁰ − 〈n_w〉³⁶⁰.

Mesophile					Thermophile
Protein	${\mu }_{ex}^{pw}\left(\text{kcal}/\text{mol}\right)$	Δµex (kcal/mol)	〈nw〉	〈Δnw〉	Protein	${\mu }_{ex}^{pw}\left(\text{kcal}/\text{mol}\right)$	Δµex (kcal/mol)	〈nw〉	〈Δnw〉
1B8P	-14.3(0.1)	-5.3(0.2)	15	8	1BDM	-15.0(0.1)	-6.0(0.2)	16	10
1GCI	-12.6(0.1)	-3.6(0.2)	16	8	1THM	-13.7(0.1)	-4.7(0.2)	16	6
3H1G	-13.2(0.1)	-4.2(0.2)	1	0	1DZ3	-24.9(0.1)	-15.9(0.2)	1	0
1IOH	-10.0(0.1)	-1.0(0.2)	13	10	3MDS	-11.7(0.1)	-2.7(0.2)	13	8
3TL2	-14.0(0.1)	-5.0(02)	9	5	1A5Z	-19.0(0.1)	-10.0(0.2)	7	3
1P3J	-14.1(0.1)	-5.1(0.2)	5	4	1ZIN	-16.4(0.1)	-7.4(0.2)	5	3
2X8S	-14.2(0.1)	-5.2(0.2)	49	24	3CU9	-14.5(0.1)	-5.5(0.2)	25	13
1GV1	-12.7(0.1)	-3.7(0.2)	130	65	4CL3	-15.94(0.1)	-3.7(0.2)	135	67