FIG 4 .

KEGG orthologs of the iron and phosphate ATP-binding cassette (ABC). Transport pathways differ between blood-fed and L. infantum-infected sand flies. (A to C) Box and whisker plots illustrate the predicted metagenomic content of ABC transport orthologs defined in KEGG. The functional metagenomes were calculated from OTUs with PICRUSt, and the ortholog content in the microbiomes of midguts from uninfected versus L. infantum-infected blood-fed sand fly midguts was determined by PICRUSt. Significant orthologs were selected by one-way ANOVA and the Tukey-Kramer posttest. All statistically significant pathways were determined using one-way ANOVA and the Tukey-Kramer posttest (*, P < 0.05). Data for uninfected and L. infantum-infected blood-fed sand flies include all samples from 1 to 12 dpi. Sequence data were obtained from the same 3 experiments described for Fig. 1.