TABLE 1 .

Experimental design showing the conditions and time points at which sand fly midgut pools were collected for sequencing^a

Condition	No. of midguts × no. of samples at indicated day postinfection
	0	1	3	6	9	12b
Sucrose fed	20 × 3c	20 × 3	20 × 3
Blood fed		20 × 3	20 × 3	20 × 3	20 × 3	20 × 2
L. infantum infected		20 × 3	20 × 3	20 × 3	20 × 3	20 × 2

^aEach cell of the table indicates the number of midguts collected under that condition at that time × the number of replicate samples taken. The full table indicates the design for each one of the three independent experiments. All replicate samples were sequenced individually, resulting in 9 sequences per condition at all time points but day 12.

^bLimited by survival/availability, two of three independent experiments included day 12 samples. For the blood-fed condition, two replicates per experiment were collected; for the infected-blood-fed condition, one and two replicates, respectively, were collected in the two experiments.

^cTwenty midguts were pooled into three independent biological replicates per time point for each condition.