. 2016 Dec 22;6(4):521–530. doi: 10.15171/apb.2016.066

Table 1. VEN- induced cytotoxicity toward isolated rat hepatocytes .

Addition	Cytotoxicity%
Incubation time (min)	60	120	180
Control	18±2	22±1	24±2
+ VEN 2mM	41±2^*	54±3^*	69±4^*
+NAC 200 µM	21±2^**	23±2^**	25±3^**
+ Taurine 200 µM	24±2^**	27±3^**	30±1^**
+ Quercetine500µM	18±1^**	24±2^**	26±2^**
+ α-Tocopherol succinate 100μM	18±1^**	24±2^**	29±2^**
+ BHT50μM	23±2^**	28±2^**	34±3^**
+ L-Carnitine2mM	28±1^**	34±2^**	40±2^**
+ Trifluperazine15µM	25±3^**	29±2^**	33±4^**
+ Fructose1mM	27±2^**	30±3^**	39±3^**
+ L-Glutamine 1mM	28±3^**	35±2^**	42±3^**
+3-Methyladenine 5mM	25±2^**	29±2^**	34±3^**
+ Chloroquine 100μM	24±2^**	28±2^**	31±3^**
+ 4-Methylpyrazole 500μM	30±2^**	38±2^**	42±3^**
+Cimetidine 1 mM	44±2	68±2^**	88±4^**
GSH depleted hepatocytes	20±2	24±2	26±2
+VEN 2mM	55±2^**	72±2^**	89±4^**

The Trypan blue exclusion test was used to assess membrane lysis rates induced by VEN. At different time points, the viability of aliquots of 1 ml (10⁶ cells) was evaluated microscopically. Hepatocytes were incubated in Krebs–Henseleit buffer, pH 7.4, at 37 °C. GSH depleted hepatocytes were prepared as described by Abdoli et.al.(Abdoli et al., 2013) Data are given as mean ± SEM for at least three independent experiments.^*Significant as compared with the control (Only hepatocytes) group (p < 0.05). **Significant as compared with VEN-treated cells (p < 0.05).