Table 2. VEN- induced ROS formation toward isolated rat hepatocytes .
| Addition | ROS formation (DCF: fluorescent intensity units) | ||
| Incubation time (min) | 15 | 30 | 60 |
| Control | 95±7 | 109±10 | 138±10 |
| + VEN 2mM | 99±7* | 116±8* | 144±10* |
| +NAC 200 µM | 98±8** | 119±10** | 156±11** |
| + Taurine 200 µM | 98±8** | 118±10** | 145±12** |
| + Quercetine500µM | 102±10** | 110±10** | 138±10** |
| + α-Tocopherol succinate 100μM | 104±6** | 120±8** | 157±10** |
| + BHT50μM | 108±7** | 123±11** | 169±9** |
| + L-Carnitine2mM | 103±7** | 125±10** | 170±10** |
| + Trifluperazine15µM | 108±6** | 123±9** | 172±10** |
| + Fructose1mM | 108±6** | 123±9** | 172±10** |
| + L-Glutamine 1mM | 110±8** | 126±9** | 178±9** |
| +3-Methyladenine 5mM | 110±8** | 126±9** | 178±9** |
| + Chloroquine 100μM | 105±8** | 125±10** | 173±8** |
| + 4-Methylpyrazole 500μM | 107±7** | 132±6** | 174±10** |
| +Cimetidine 1 mM | 130±5** | 189±10** | 268±10** |
| GSH depleted hepatocytes | 92±6 | 118±10 | 142±10 |
| +VEN 2mM | 139±9** | 200±12** | 284±10** |
Hepatocytes were incubated in Krebs–Henseleit buffer, pH 7.4, at 37 °C for 1.0 h after VEN (2mM) treatment. Dichlorofluorescein (DCF) formation was measured as fluorescent intensity units. GSH depleted hepatocytes were prepared as described by Abdoli et.al.(Abdoli et al., 2013) Data are given as mean ± SEM for at least three independent experiments.
*Significant as compared with the control (Only hepatocytes) group (p < 0.05).
**Significant as compared with VEN-treated cells (p < 0.05).