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. 2016 Dec 22;6(4):563–571. doi: 10.15171/apb.2016.070

Figure 4.

Figure 4

Binding of three selected scFvs clones with high reactivity to EGFRvIII. The reactivity of different dilutions of scFv-2, scFv-15, and scFv- 10 with EGFRvIII was assessed by indirect ELISA. The EGFRvIII peptide with concentration of 25μg/ml was coated on ELISA plates and binding of the produced scFvs in TG1 were detected with Protein L conjugated to HRP at 1:2000 dilutions. Absorbance values are represented as the mean ± SD for three independent determinations. Error bars show the standard deviation for each set of data.