. 2016 Dec 22;6(4):627–637. doi: 10.15171/apb.2016.077

Table 2. MMP changes induced by sumatriptan in rat liver hepatocytes and protective effect of antioxidants radical scavengers, lysosomal membrane stabilizers, mitochondrial ATP generators, MPT pore sealing agents and CYP450 inhibitors .

Treatment	MMP (% of control)
	Incubation time
	15 min	30 min	60 min
Control (intact hepatocytes)	100	97 ± 3	92 ± 3
Sumatriptan (5 mM)	72 ± 3^a	65 ± 2^a	53 ± 2^a
+Taurine (200 µM)	89 ± 3^b	80 ± 3^b	73 ± 2^b
+Quercetin (500 µM)	85 ± 3^b	78 ± 3^b	70 ± 3^b
+NAC (200 µM)	90 ± 3^b	81 ± 3^b	75 ± 2^b
+Vitamin E (100 µM)	85 ± 3^b	79 ± 2^b	69 ± 3^b
+BHT (50 μM)	86 ± 2^b	80 ± 3^b	74 ± 3^b
+Methylamine (30 mM)	89 ± 2^b	83 ± 3^b	79 ± 2^b
+Chloroquine (100 μM)	84 ± 3^b	76 ± 2^b	68 ± 2^b
+Fructose (1 mM)	85 ± 3^b	77 ± 3^b	70 ± 3^b
+L- Gln (1 mM)	87 ± 3^b	77 ± 2^b	69 ± 3^b
+ TFP (15 µM)	87 ± 3^b	81 ± 2^b	77 ± 2^b
+Carnitine (2 mM)	86 ± 2^b	77 ± 3^b	65 ± 2^b
+4- MP (500 µM)	88 ± 3^b	82 ± 3^b	77 ± 2^b
+Cimetidine (2 mM)	86 ± 3^b	81 ± 3^b	76 ± 2^b
GSH depleted hepatocytes	98 ± 2	95 ± 2	93 ± 3
+ Sumatriptan (5 mM) (in GSH depleted hepatocytes)	61 ± 3^c	53 ± 2^c	41 ± 2^c

MMP was determined as the percentage of mitochondrial rhodamine 123 reuptake between control and treated cells. Results are expressed as mean ± S.E. of three separate experiments.

^a Significantly lower than control (p < 0.05).

^bSignificantly higher than sumatriptan treated hepatocytes (p < 0.05).

^cSignificantly lower than sumatriptan treated hepatocytes (in comparison with GSH depleted cells) (p < 0.05).