Table 4. Lipid peroxidation induced by sumatriptan in rat liver hepatocytes and protective effect of antioxidants, radical scavengers, lysosomal membrane stabilizers, mitochondrial ATP generators, mitochondrial permeability transition pore sealing compounds and CYP450 inhibitors .
| Treatment | TBARS (μM per 10 6 cells) | ||
| Incubation time | |||
| 15 min | 30 min | 60 min | |
| Control (only hepatocytes) | 0.082 ± 0.005 | 0.091 ± 0.005 | 0.144 ± 0.009 |
| Sumatriptan (5 mM) | 0.149 ± 0.012 a | 0.181 ± 0.013 a | 0.245 ± 0.024 a |
| +Taurine (200 µM) | 0.109 ± 0.007 b | 0.127 ± 0.009 b | 0.176 ± 0.011 b |
| +Quercetin (500 µM) | 0.118 ± 0.011 b | 0.141 ± 0.013 b | 0.196 ± 0.010 b |
| +NAC (200 µM) | 0.105 ± 0.010 b | 0.123 ± 0.009 b | 0.178 ± 0.011 b |
| +Vitamin E (100 µM) | 0.115 ± 0.008 b | 0.147 ± 0.011 b | 0.197 ± 0.014 b |
| +BHT (50 μM) | 0.114 ± 0.010 b | 0.140 ± 0.007 b | 0.192 ± 0.011 b |
| +Methylamine (30 mM) | 0.119 ± 0.011 b | 0.141 ± 0.013 b | 0.198 ± 0.013 b |
| +Chloroquine (100 μM) | 0.116 ± 0.007 b | 0.144 ± 0.010 b | 0.202 ± 0.010 b |
| +Fructose (10 mM) | 0.120± 0.007 b | 0.149 ± 0.011 b | 0.191 ± 0.011 b |
| +L-Gln (1 mM) | 0.117 ± 0.007 b | 0.137 ± 0.010 b | 0.197 ± 0.012 b |
| + TFP (15 µM) | 0.113 ± 0.010 b | 0.140 ± 0.014 b | 0.201± 0.010 b |
| +Carnitine (2 mM) | 0.119 ± 0.011 b | 0.146 ± 0.008 b | 0.192± 0.010 b |
| +4- MP (500 µM) | 0.112 ± 0.009 b | 0.135 ± 0.009 b | 0.195 ± 0.011 b |
| +Cimetidine (2 mM) | 0.120 ± 0.009 b | 0.138 ± 0.010 b | 0.204 ± 0.011 b |
| GSH depleted hepatocytes(control) | 0.097 ± 0.008 | 0.124 ± 0.007 | 0.163 ± 0.005 |
| + Sumatriptan (5 mM) (in GSH depleted hepatocytes) | 0.186 ± 0.010 c | 0.239 ± 0.011 c | 0.357 ± 0.019 c |
TBARS formation was expressed as µM concentrations. Results are expressed as mean ±S.E. of three separate experiments.
a Significantly higher than control (p < 0.05).bSignificantly lower than sumatriptan treated hepatocytes (p < 0.05).cSignificantly higher than sumatriptan treated hepatocytes (p < 0.05).