Table 5. GSH depletion induced by sumatriptan in rat liver hepatocytes and protective effect of antioxidants and radical scavengers, lysosomal membrane stabilizers, mitochondrial ATP generators, MPT pore sealing compounds and CYP450 inhibitors .
| Treatment | Intracellular GSH (nmol per 10 6 cell) | ||
| Incubation time | |||
| 60 min | 120 min | 180 min | |
| Control (intact hepatocytes) | 57.1 ± 1.2 | 51.5 ± 2.1 | 48.3 ± 1.6 |
| Sumatriptan (5 mM) | 46.3 ± 2.2a | 38.6 ± 1.9a | 31.6 ± 2.3a |
| +Taurine (200 µM) | 56.4± 2.6b | 49.8± 3.3b | 46.8± 3.1b |
| +Quercetin (500 µM) | 51.3± 2.8b | 48.9± 2.8b | 47.3± 2.1b |
| +NAC (200 µM) | 52.1± 3.1b | 49.3± 3.5b | 47.1± 2.1b |
| +Vitamin E (100 µM) | 51.2± 3.3b | 49.2± 3.6b | 47.3± 2.2b |
| +BHT (50 μM) | 52.8± 2.4b | 48.2± 2.8b | 45.9± 2.7b |
| +Methylamine (30 mM) | 53.5± 2.2b | 47.6± 3.1b | 43.7± 2.1b |
| +Chloroquine (100 μM) | 51.6± 3.2b | 46.5± 3.7b | 43.1± 3.6b |
| +Fructose (10 mM) | 52.2± 3.1b | 48.7± 2.9b | 44.7± 3.5b |
| +L- Gln (1 mM) | 53.4± 3.5b | 48.6± 3.7b | 45.3± 3.9b |
| + TFP (15 µM) | 51.5± 2.8b | 47.9± 3.6b | 44.9± 3.7b |
| +Carnitine (2 mM) | 54.2± 2.5b | 48.3± 3.8b | 45.7± 2.6b |
| +4- MP (500 µM) | 53.3± 3.4b | 48.4± 2.6b | 44.6± 3.3b |
| +Cimetidine (2 mM) | 52.2± 3.3b | 48.1± 2.3b | 43.3± 3.2b |
Results are expressed as the means ± S.E of three separate experiments.
a Significantly lower than control (p < 0.05).b Significantly higher than sumatriptan treated hepatocytes (p < 0.05).