Figure 8.

Expression of catabolic markers on chondrocytes cultured with three different conditions for 5 days and determined by western blot ((a), (b), (d), and (e)) and real-time PCR ((c), (f)). Caspase-3 protein was downregulated in chondrocytes when they were cultured with ADSCs cocultured system and TGF-β1-containing medium ((a), (b)). The mRNA level of caspase-3 was also decreased in chondrocytes cultured with ADSCs cocultured system and TGF-β1-containing medium (c). Furthermore, both MMP3 and MMP13 were suppressed in chondrocytes cultured with ADSCs cocultured system and TGF-β1-containing medium tested by western blot ((d), (e)) and real-time PCR (f). Data are shown as mean ± SEM. ^∗∗ p < 0.01 versus chondrocytes group.