Fig. 2.

Validation of AMPK isoform-specific antibodies for IP. A–E: Endogenous AMPK complexes were immunoprecipitated using the indicated antibodies from 200 μg extract protein from the gastrocnemius muscle (A and C) or liver (B, D, and E) of the indicated AMPK WT or KO mice. IB analysis was performed on the input (10% = 20 μg), the supernatant (SN; 10% = 20 μg), and the immunoprecipitates (IP) with the indicated antibodies. F: summary table of the isoform-specific antibodies used in the present study.