Fig. 3.

Effect of the small-molecule AMPK activator 991 on activity of a panel of 139 protein kinases. A: a kinase screen was performed by the International Centre for Kinase Profiling (University of Dundee). Assays were performed in the presence of 1 μM 991. Results are shown in rank order as mean percent activity compared with controls in the absence of compound ± SD. B–E: mouse embryonic fibroblasts were treated with vehicle (0.1% DMSO) or 991 (10 μM) for 1 h (3 independent plates for each condition). Cell lysates were immunoblotted with the indicated antibodies (B). The indicated kinases were immunoprecipitated from cell lysates, and in vitro kinase activity toward substrate peptide was measured (C–E) as described in materials and methods. ACC, acetyl-CoA carboxylase; RAPTOR, regulatory associated protein of mechanistic target of rapamycin; SIK3, salt-inducible kinase. Results are expressed as means ± SD. *P < 0.05.