Fig. 1.

Generation of C1q/TNF-related protein 1 (Ctrp1) knockout (KO) mice. A: schematic showing the strategy for generating Ctrp1 KO mice by targeted deletion of exon 4 of the mouse Ctrp1 gene and replacement with a lacZ reporter and a neomycin resistance cassette. The red triangle represents the FRT site recognized by Flp recombinase. UTR, untranslated region. B: genotyping results indicate the successful generation of Ctrp1 wild-type (WT; +/+), heterozygous (+/−), and homozygous KO (−/−) alleles using the primer set (forward and reverse arrows) indicated in A. C: semiquantitative PCR analysis indicating the absence of detectable Ctrp1 mRNA in KO mice. D: serum CTRP1 levels of low-fat diet (LFD)-fed WT and KO male mice. All data are expressed as means ± SE. ****P < 0.0001.