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. 2017 Jan 18;7:40884. doi: 10.1038/srep40884

Figure 2. Identification of novel miRNAs and evidence for miRNA arm switch in A. mellifera embryogenesis.

Figure 2

(a) Localization of novel miRNAs in the precursor sequences. Secondary structure of honey bee miRNAs precursors (retrieved from miRBase version 20) were generated by RNAfold. Mature miRNAs 3p and 5p are highlighted in the secondary structure. (b) Amplification of novel miRNAs by RT-qPCR. cDNA from female embryo and worker larvae were used to validate the novel miRNAs found in the small RNAs libraries of honey bee embryos. (c) Table of number of reads mapped to each arm of miRNAs that showed arm switching during honey bee embryogenesis (d) Expression profiles of ame-miR-3759, ame-miR-317 and ame-miR-318 showed alternated dominance of the 5p and 3p arms in honey bee embryogenesis.