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. 2017 Jan 18;7:40681. doi: 10.1038/srep40681

Figure 4. The effects of IFN-γ on the odonto/osteogenic differentiation of DPSCs.

Figure 4

DPSCs were cultured in odonto/osteogenic medium containing IFN-γ at different concentrations for 2 weeks with or without the MAPK inhibitor (SB203580: 20 μΜ, U0126: 25 μΜ, SP600125: 25 μΜ), or NF-κB inhibitor (PDTC: 20 μΜ). Alizarin red staining and alizarin red quantification were used to evaluate the formation of calcium nodules (a–d). The protein expression of ALP, DSPP, OCN and β-actin was analysed by Western blotting (e, Supplementary Fig. 1a). The relative band intensities were determined by densitometry (f). Statistical analysis was performed using one-way ANOVA. Data are shown as the mean ± SD. *P < 0.05 when compared with the control. #P < 0.05 when compared with the IFN-γ group. Scale bars = 100 μm.