Figure 5. Involvement of NF-κB and MAPK signaling pathways in the IFN-γ-induced inhibition odonto/osteogenic differentiation of DPSCs.

DPSCs were cultured in odonto/osteogenic medium containing 0.5 ng/ml IFN-γ with or without MAPK inhibitor (SB203580: 20 μΜ, U0126: 25 μΜ, SP600125: 25 μΜ), or NF-κB inhibitor (PDTC: 20 μΜ). The protein expression of P65, p-P65, P38, p-P38, ERK, p-ERK, JNK, p-JNK was analysed by Western blotting (a, Supplementary Fig. 1b). The relative band intensities were determined by densitometry (b–e). Statistical analysis was performed by using one-way ANOVA. Data are shown as means ± SD. *P < 0.05 when compared with the control group. ^#P < 0.05 when compared with the IFN-γ 60 min group.