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. 2017 Jan 18;7:40787. doi: 10.1038/srep40787

Figure 3. LFAO-seeded Aβ aggregates display distinct biophysical characteristics.

Figure 3

(a) ThT fluorescence aggregation kinetics of Aβ monomer (50 μM) in the absence of seed (Un, □) or seeded with 10 μM LFAOs (sLFAO, ●) or fibrils (sFib, △). (b) Denaturing (SDS) PAGE of Un (1), sFib (2), and sLFAO (3) at 14 days of incubation. The single and double arrows represent LFAO 12 (56 kDa) and 24mer (110 kDa), respectively. (c) Non-denaturing (native) PAGE of parent monomer (4), fibril (5), and LFAO (6) along with Un (7), sFib (8), and sLFAO (9). The lower, middle, and top dashed lines represent native migration of LFAO 12mer, 24mer, and HMW oligomeric species, respectively. 540 ng was loaded into each well for all samples. (d) Percentage of solubility, as determined by centrifugation and ThT fluorescence, at 28 days of incubation (14 days at 25 °C, 14 days at 4 °C). (e–f) Quantitative SEC of sLFAO (e) and sFib (f) at 4, 5, 6, and 7 days of incubation, respectively.