Figure 1. Morphology of FFPE HER2 positive, grade 3 IDC breast cancer tissue.

Tissue sections were stained with H&E and overview images were collected at 5X magnification to show tumor morphology (A, D and G). The box in the H&E image represents the field-of-view of the adjacent immunofluorescence images. Scale bars for H&E images = 200 μm. Images of immunofluorescence staining were collected at 10X magnification to demonstrate the tissue patterns of HER2, TOM20, and Lamin B1 proteins (B, E and H). Scale bars = 50 μm. Additional images were collected at 40X magnification to enhance visualization of ultrastructural features of each immunostain (C, F and I), scale bars = 20 μm.