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(A) Representative astigmatic single-molecule images collected on 2 and 4 μm sections. (B) Reconstructed 3D SMLM image of a 4 μm FFPE tumor section immunolabeled for HER2, with z-positions color coded (see legend). The bottom image shows the zoomed-in view of the boxed area in the top image. Scale bars, 5 μm (left), 10 μm (right, top) and 2 μm (right, bottom).
