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. 2017 Jan 18;7:40756. doi: 10.1038/srep40756

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Copyright © 2017, The Author(s)

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(A) Up: schematic representation of the bioUFM1 vector for mammalian cells. Below, left: Validation of HA-DDRGK. Right, top twenty bioUFM1-modified proteins. (B) GO analysis for biological process, cellular component and molecular function of the selected bioUFM1-conjugated protein set (n = 82). (C) Validation of bioUFM1-modified proteins CYB5R3 and PSMB5 fused to HA tag. In the elution panels (lanes 3 and 4), black arrowheads indicate the modified forms of the respective proteins. Residual non-specific interactions of non-modified forms are indicated by white arrowheads. Molecular weight markers are shown to the left.