Table 3. Primers sequences and their parameters used in this study.
| Gene/Assay | Forward primer 5′–3′ | Reverse primer 5′–3′ | Amplicon size bp/annealing temperature °C/No. of CpGs in amplicon* | Genbank accession number |
|---|---|---|---|---|
| SNRPN qRT-PCR | CGAATCTTCATTGGCACCTT | AGCAACACCAGACCCAAAAC | 143/60 | NM_003097.3 |
| PEG10 qRT-PCR | CAAGCCACCACCAGGTAGAT | GAGGCACAGGTTCAGCTTTC | 101/62 | NM_001040152.1 |
| MEST qRT-PCR | TGGGCTTCATCAACTCCTTC | AGGACCTCTTTTGGGCATTT | 112/62 | NM_001253900.1 |
| GAPDH qRT-PCR | CGACCACTTTGTCAAGCTCA | AGGGGTCTACATGGCAACTG | 228/60-65 | NM_001256799.2 |
| SNRPN MS-HRM | GTTATCGGTATAGTTGATTTTGTT | AAACCTACCGCTACTACAAC | 123/58/12 | U41384.1 |
| PEG10 MS-HRM | GGTTTATAGTTTGCGTTTTTGGTAT | CGATCTCCACTAAATACTCC | 176/58/16 | NG_011340.1 |
| MEST MS-HRM | GAAGGCGGTAGTATATGTTGGGT | CGACCTTCACCCTATTCCCAAAA | 118/62/13 | NG_009226.1 |
| SNRPN qChIP-PCR | CCTCTGAACATTCCGGATCTG | AACGGAATTTGGGCCCTAAA | 60/60 | U41384.1 |
| PEG10 qChIP-PCR | CCGTCCGTCCTCGATTCTC | GCTAGAGGGAGTACGGGATTACC | 108/62 | NG_011340.1 |
| MEST qChIP-PCR | CTCGTGCCCTTGGTGGTTA | GCCACGAGGGCCCTATG | 57/62 | NG_009226.1 |
*In case of only MS-HRM assays. qRT-PCR: quantitative Reverse transcriptase polymerase chain reaction. MS-HRM: Methylation- sensitive high resolution melting. qChIP: quantitative Chromatin immunoprecipitation assay.