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. 2016 Nov 21;292(2):407–416. doi: 10.1074/jbc.M116.766832

FIGURE 1.

FIGURE 1.

PTP1B overexpression inhibits VEGF-induced phosphorylation of VEGFR2 and Akt. ALLN further increased VEGF-induced VEGFR2 phosphorylation via inhibition of calpain/PTP1B axis, which is however absent in the presence of PTP1B overexpression. Bovine aortic endothelial cells (BAECs) were transfected with wild type PTP1B (HA-PTP1B) or empty vector (pCMV-HA). The cells were treated with ALLN (30 μm) for 30 min prior to VEGF stimulation (100 ng/ml, 5 min). A, representative Western blots of indicated proteins. B and C, grouped densitometric data after normalization to total-VEGFR2 or β-actin (n = 4). PTP1B overexpression inhibited VEGF-induced phosphorylation of VEGFR2 and Akt. *, p < 0.05; **, p < 0.01. NS, not significant.