Percentage of cancer cells expressing stem cell marker CD133 and D2 DA receptors in human NSCLC adenocarcinoma cell lines (A549 and NCI-H23).
A and B, forward scatter/side scatter (FSC/SSC) plots show the gated population. C, unstained A549 cells as a control. D, percentage of A549 cells expressing D2 DA receptors. E, unstained NCI-H23 cells as a control. F, percentage of NCI-H23 cells expressing D2 DA receptors. G, unstained A549 cells as a control. H, percentage of A549 cells expressing CD133. I, unstained NCI-H23 as a control. J, percentage of NCI-H23 cells expressing CD133. K, unstained A549 cells as a control. L, A549 cells grouped by double staining of CD133 and D2 DA receptors. M, unstained NCI-H23 as a control. N, NCI-H23 cells grouped by double staining CD133 and D2 DA receptors. Results are representative of 3 independent experiments.