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. 2016 Nov 23;292(2):462–476. doi: 10.1074/jbc.M116.744367

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FIGURE 2. — Expression, purification, and biophysical characterization of recombinant basigin. A, SDS-PAGE analysis of the purified histidine-tagged recombinant basigin. The purified recombinant protein was resolved on 12% SDS-PAGE. Lane 1, purified histidine-tagged basigin. Lane M, molecular weight markers. B, circular dichroism spectra of purified histidine-tagged recombinant basigin. C, gel permeation chromatography profile of purified histidine-tagged basigin. Elution profile of molecular weight markers and purified histidine-tagged basigin are represented by gray and black lines, respectively.