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. 2016 Nov 23;292(2):462–476. doi: 10.1074/jbc.M116.744367

FIGURE 4.

FIGURE 4.

Assessment of interaction between basigin and PvTRAg38 by yeast two-hybrid assay. A, co-transformed yeast lines growing on different dropout media agar plates. Growth of transformed and parent strain yeast cells was on a YPD agar plate (panel a) and on an SD/Trp−Leu−His−Ade− dropout plate with 10 mm AT (panel b), 30 mm AT (panel c), and 50 mm AT (panel d). Zones on the plates are showing growth of yeast AH109 cells co-transformed with positive control pGAD-T + pGBKT7–53 (1), test pGAD-BSG + PGBKT7-PvTRAg38 (2), AH109 cells (3), empty pGAD + PGBKT7 vectors (4), control pGAD-BSG + pGBKT7 (5), and control pGBKT7-PvTRAg38 + pGAD vector (6). B, liquid assay to measure relative β-galactosidase activity. Three independent assays and S.D. are represented by error bars.