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. 2016 Nov 23;292(2):462–476. doi: 10.1074/jbc.M116.744367

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FIGURE 5. — Assessment of basigin and PvTRAg38 interaction by mammalian expression system. The pRE4-basigin transfected HEK-293 cells expressing basigin on the surface (upper panel) or HEK-293 cells transfected with pRE4 vector alone (lower panel) were incubated with GST-tagged PvTRAg38 and then co-localized with anti-GST monoclonal and anti-basigin polyclonal antibodies. Secondary antibodies used were Alexa Fluor 488-tagged anti-mouse and Alexa Fluor 594-tagged anti-rabbit. Smears were mounted in DAPI anti-fade and sealed.