FIGURE 8.

Inhibition of P. falciparum growth by peptides P₂ and P₄ of PvTRAg38. Parasite culture at late schizont stage was incubated with peptide P₂, peptide P₄, peptides P₂ + P₄, PvTRAg38, PvTRAg38.7, and other controls. After 40 h, parasitemia was determined by ethidium bromide staining and measured by flow cytometry. A, bar diagram shows the percentage of parasite growth in the absence or presence of PvTRAg38 and its derived peptides at different concentrations (0–50 μm). Data shown are the mean ± S.D. for two triplicate experiments. B, representative dot plots showing percent infection. Panel a, infected erythrocytes treated with PBS. Panel b, infected erythrocytes treated with peptide P₄ (20 μm). Panel c, infected erythrocytes treated with peptide P₂ (20 μm). Panel d, infected erythrocytes treated with 20 μm peptide P₂ and peptide P₄. Panel e, infected erythrocytes treated with PvTRAg38 (20 μm), and panel f, infected erythrocytes treated with PvTRAg38.7 (20 μm).