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pH dependence of net charge on α-LA (blue) and lysozyme (red), calculated as described in text using amino acid sequences obtained from Protein Data Bank data files 1HFZ (bovine α-lactalbumin) and 5KXK (hen egg lysozyme). The isoelectric points (pI) of α-LA and lysozyme, indicated by X marks on the plot, are estimated to be ∼4.9 and ∼9, respectively.
