TABLE 2.
Thermodynamic analysis by ITC of interactions between RCD1-RST(499–572) and different transcription factors
All experiments were performed as described under “Experimental Procedures.” Syringe/cell indicates whether RCD1-RST or the transcription factor is the titrant in syringe or the titrant in cell. The standard errors for ΔH, Kd, and N were obtained from Origin when fitting the data to a one set of sites binding model.
| Protein | Kd | N | ΔH | −TΔS | ΔG | Syringe/cell |
|---|---|---|---|---|---|---|
| nm | kJ/mol | kJ/mol | kJ/mol | |||
| ANAC016(325–367) | 200 ± 76 | 1.02 ± 0.04 | −42.5 ± 2.3 | 4.2 | −38.3 | ANAC016/RCD1 |
| ANAC017(296–339) | 37 ± 9 | 0.89 ± 0.02 | −54.2 ± 1.0 | 11.7 | −42.5 | ANAC017/RCD1 |
| bZIP23(15–36) | 128 ± 40 | 0.94 ± 0.05 | −30.6 ± 2.1 | −8.7 | −39.3 | RCD1/bZIP23 |
| STO(229–248) | 90 ± 90 | 1.16 ± 0.12 | −3.8 ± 0.5 | −36.3 | −40.1 | RCD1/STO |
| COL10(175–208) | 418 ± 201 | 1.04 ± 0.08 | −9.2 ± 1.0 | −27.2 | −36.4 | RCD1/COL |
| bHLH19(271–295) | NBa | RCD1/bHLH19 | ||||
| IRL3(69–100) | NB | RCD1/IRL3 | ||||
| MYB91(100–291) | NB | RCD1/MYB91 | ||||
| MYB(100–230) | NB | RCD1/MYB91 | ||||
| MYB92(239–267) | NB | RCD1/MYB91 | ||||
| MYB91(239–291) | NB | RCD1/MYB91 |
a NB means no detectable binding.