TABLE 3.
Transduction ratios log(τ/KA) of CCL4, UCB35625, and J113863 at CCR5
Data were analyzed by non-linear regression using the operational model equation to determine the transduction ratios (log(τ/KA)). Δlog(τ/KA) ratios were calculated from log(τ/KA) values considering CCL4 as the reference ligand. The RE of ligands toward each pathway, relative to CCL4, corresponds to the inverse logarithm of the Δlog(τ/KA) ratio. Data are the mean ± S.E. of 3–6 experiments. ND, not determined.
| Ligand | Gi2 |
Goa |
Ca2+ |
β-Arrestin 2 |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Log | ΔLog | RE | Log | ΔLog | RE | Log | ΔLog | RE | Log | ΔLog | RE | |
| CCL4 | 8.68 ± 0.44 | 0.00 ± 0.63 | 1 | 8.65 ± 0.21 | 0.00 ± 0.29 | 1 | 7.88 ± 0.19 | 0.00 ± 0.27 | 1 | 8.15 ± 0.09 | 0.00 ± 0.12 | 1 |
| UCB35625 | 5.88 ± 0.21 | −2.81 ± 0.49 | 0.0016a | 5.05 ± 0.18 | −3.60 ± 0.27 | 0.0002a | ND | ND | ND | ND | ND | ND |
| J113863 | 7.10 ± 0.72 | −1.58 ± 0.84 | 0.0261 | 5.23 ± 0.32 | −3.42 ± 0.42 | 0.0004a | 4.43 ± 0.44 | −3.46 ± 0.47 | 0.0003a | ND | ND | ND |
a p < 0.05.