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. 2016 Dec 1;292(2):652–660. doi: 10.1074/jbc.M116.761528

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Interaction of BCAP and other TIR domain-containing proteins. HEK 293T cells were overexpressed with Myc-TRAM, FLAG-BCAP, Myc-BCAP, FLAG-SARM, Myc-MyD88, and a Myc-MyD88-FXXM mutant as indicated. Cells were lysed, and immunoprecipitation (IP) was performed using anti-FLAG antibody. Precipitates were split and assayed for the presence of FLAG- or Myc-tagged proteins. A, BCAP shows no interaction with TRAM (lane 1, Myc-TRAM+FLAG-BCAP-FL; lane 2, Myc-TRAM). WCL, whole cell lysate. B and C, BCAP interacts with SARM (lane 1, Myc-BCAP-FL; lane 2, Myc-BCAP-FL and FLAG-SARM) (B) and with both MyD88 and the FXXM mutant of MyD88 (lane 1, Myc-MyD88-WT and FLAG-BCAP-FL; lane 2, Myc-MyD88-WT; lane 3, Myc-MyD88-FXXM and Myc-BCAP-FL; lane 2, Myc-MyD88-FXXM) (C). HC and LC are the heavy and light chains of the mouse anti-FLAG antibody, respectively.