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. 2016 Dec 1;292(2):661–671. doi: 10.1074/jbc.M116.749804

FIGURE 2.

FIGURE 2.

The titers of natural antibodies are reduced in the CD6−/− mice. Sera from naïve WT and CD6−/− mice were analyzed for total IgG (A) and total IgM (B) by ELISA. Data are representative of mIgM WT (n = 30), CD6−/− (n = 36), mIgG WT (n = 17), and CD6−/− (n = 24). Sera from WT, CD6−/−, and CD6−/− mice supplemented with WT IgM used in the intestinal I/R-induced injury studies were analyzed for annexin IV (C) and PC-specific IgM levels (D) by ELISA. Data are representative of WT (n = 14), CD6−/− (n = 18), and CD6−/− + IgM (n = 9). E, the local jejunum tissue lysates levels of IL-6 in the WT, CD6−/−, and CD6−/−+IgM after intestinal I/R-induced injury were quantified by ELISA. Data are representative of WT (n = 14), CD6−/− (n = 18), and CD6−/− + IgM (n = 9). *, p < 0.01; **, p < 0.001; ***, p < 0.0001. NS, not significant.