FIGURE 2.

DJ-1β knock-out flies do not have elevated levels of AGEs. A–B, DJ-1β knock-out flies do not have elevated levels of AGEs. Flies were grown under density controlled conditions and aged 6 weeks to allow accumulation of MG adducts. MG-adducts were detected either with an antibody that recognizes a variety of MG adducts (AGE, panel A), or specifically CEL (panel B). C, DJ-1β knock-out mutation was crossed into Glo1[BG02656] hypomorphic mutants, which have strongly reduced levels of Glo1 protein, detected by immunoblotting.