FIGURE 10.

Up-regulation of differentially expressed FOXP3 and EZH2 targets indicates deregulation of a FOXP3-EZH2 pathway in Crohn's disease. A, heatmap of differential expression of 275 gene targets co-regulated by EZH2 and FOXP3. 187 of 275 gene targets were up-regulated. Shown is the rank order of ingenuity pathway application canonical pathways of up-regulated (top bracket) and down-regulated (bottom bracket) genes of patients compared with control subjects. B, ingenuity pathway analysis demonstrating the top enriched canonical pathways (x axis), the enrichment of DEGs within each pathway (y axis), and the status as up-regulation (red) or down-regulation (green). C, the ratio of TF (FOXP3, TBX21, or GATA3) and EZH2 common targets over non-common targets represented within the set of DEGs were compared using chi-squared test. Although the DEGs were significantly enriched for the common targets of FOXP3 and EZH2, there was no significant difference for the common targets of either TBX21 or GATA3 with EZH2.